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Building more sustainable, equitable, and resilient food systems means rethinking how we consume, produce, and safeguard agrobiodiversity that can benefit the planet and secure access to nutritious food for all. This was the purpose of the 2021 Second International Agrobiodiversity Congress, convening scientists, Indigenous Peoples, entrepreneurs, and policymakers to share and advance research, nature-positive solutions, and policies. Congress organizers set out to showcase agrobiodiversity’s role in positively transforming food systems, present solutions and business opportunities to enhance multi-stakeholder collaborations, and reinforce the commitments made during international events of 2021. The take-home messages from this Congress support the 2021 UN Food Systems Summit to chart a path of concrete actions that can deliver on the 2030 Agenda for Sustainable Development