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The quantitative evaluation of the suitability of land fallow is of great significance to the effective implementation of fallow system in rural China. The purpose of this study is to systematically evaluate the cultivated areas suitable for fallow in Chongqing, China. The results show that: (1) a comprehensive index of cultivated land fallow (ILF) was developed by employing a series of multi—source data, and the ILF has been proven as an effective proxy to identify the cultivated areas suitable for fallow; (2) cultivated land with ILF values above the average value accounts for 34.38% (9902 km2) of the total cultivated land; (3) the ILF is negatively correlated with the population density, transportation proximity, and proportion of inclined area. This study argued that the ILF can reflect the cultivated areas suitable for fallow in Chongqing and can provide guidance for the spatial distribution of cultivated land fallow. The findings indicated that the differences in geographical elements between karst and non—karst areas must be further investigated, and the evaluation accuracy of the cultivated areas suitable for fallow must be improved.