In the past decades, sugarcane production in Brazil has expanded rapidly to meet increasing ethanol demand. The large majority of this expansion occurred in Sao Paulo state. We used an integrated approach considering location-specific biophysical characteristics to determine the environmental impacts of sugarcane expansion and their spatial variation in Sao Paulo state (2004–2015). The included environmental impacts are greenhouse gas (GHG) emissions, biodiversity, soil erosion, and water quantity.

Spatially explicit assessments of ecosystem services (ES) potentials are a key component in supporting a sustainable land use management. The ES matrix method is a commonly used approach as it allows for a comparably fast, comprehensible and accessible ES assessment. As it is often based on land use/land cover data (LULC) with no spatial variability, a main critique is that the results fail to assess spatial variability at landscape levels, which limits the reliability of the outputs for spatial planning applications.

Preserving landscape connectivity is one of the most frequently recommended strategies to address the synergistic threats of climate change, habitat fragmentation, and intensifying disturbances. Although assessments to develop plans for linked and connected landscapes in response to climate and land-use change have been increasingly employed in the last decade, efforts to operationalize and implement these plans have been limited. Here, we present a framework using existing, available biological data to design an implementable, comprehensive multispecies connectivity plan.